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Amgen recombinant rat stem-cell factor
Recombinant Rat Stem Cell Factor, supplied by Amgen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+rat+stem+cell+factor/recombinant+rat+stem+cell+factor/pmc05093088-175-51-62
Average 90 stars, based on 1 article reviews
recombinant rat stem-cell factor - by Bioz Stars, 2026-09
90/100 stars

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Cell Culture:

Article Title: Trichinella spiralis Secreted Enzymes Regulate Nucleotide-Induced Mast Cell Activation and Release of Mouse Mast Cell Protease 1
Article Snippet: .. Bone marrow cell cultures were established from BALB/c mice by cell culture in Dulbecco’s minimal essential medium (DMEM), 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U ml 1 penicillin, 100 g ml 1 streptomycin, 2.5 g ml 1 amphotericin B (Fungizone), 1 mM sodium pyruvate, 15% WEHI-3B-conditioned medium as a source of IL-3, 50 ng ml 1 recombinant rat stem cell factor (Amgen, Thousand Oaks, CA), 5 ng ml 1 recombinant mouse IL-9 (R&D Systems), and 1 ng ml 1 transforming growth factor 1 (TGF- 1; R&D Systems), grown for 21 days, and passaged at 7-day intervals. ..

Article Title: Trichinella spiralis Secreted Enzymes Regulate Nucleotide-Induced Mast Cell Activation and Release of Mouse Mast Cell Protease 1
Article Snippet: .. Bone marrow cell cultures were established from BALB/c mice by cell culture in Dulbecco's minimal essential medium (DMEM), 10% fetal calf serum (FCS), 2 mM l -glutamine, 100 U ml −1 penicillin, 100 μg ml −1 streptomycin, 2.5 μg ml −1 amphotericin B (Fungizone), 1 mM sodium pyruvate, 15% WEHI-3B-conditioned medium as a source of IL-3, 50 ng ml −1 recombinant rat stem cell factor (Amgen, Thousand Oaks, CA), 5 ng ml −1 recombinant mouse IL-9 (R&D Systems), and 1 ng ml −1 transforming growth factor β 1 (TGF-β 1 ; R&D Systems), grown for 21 days, and passaged at 7-day intervals. ..

Recombinant:

Article Title: Trichinella spiralis Secreted Enzymes Regulate Nucleotide-Induced Mast Cell Activation and Release of Mouse Mast Cell Protease 1
Article Snippet: .. Bone marrow cell cultures were established from BALB/c mice by cell culture in Dulbecco’s minimal essential medium (DMEM), 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U ml 1 penicillin, 100 g ml 1 streptomycin, 2.5 g ml 1 amphotericin B (Fungizone), 1 mM sodium pyruvate, 15% WEHI-3B-conditioned medium as a source of IL-3, 50 ng ml 1 recombinant rat stem cell factor (Amgen, Thousand Oaks, CA), 5 ng ml 1 recombinant mouse IL-9 (R&D Systems), and 1 ng ml 1 transforming growth factor 1 (TGF- 1; R&D Systems), grown for 21 days, and passaged at 7-day intervals. ..

Article Title: PECAM-1 is expressed on hematopoietic stem cells throughout ontogeny and identifies a population of erythroid progenitors.
Article Snippet: Platelet endothelial cell adhesion molecule-1 (PECAM-1) (CD31) is an adhesion molecule expressed on endothelial cells and subsets of leukocytes.. Analysis of phenotypically defined hematopoietic stem cells (HSCs) from the yolk sac, fetal liver, and adult bone marrow demonstrates CD31 expression on these cells throughout development.. CD31 c-kit cells, but not CD31 c-kit cells, isolated from day-9.5 yolk sac give rise to multilineage hematopoiesis in vivo.

Article Title: Evaluation of the silicon phthalocyanine pc 4 for photodynamic bone marrow purging.
Article Snippet: The silicon phthalocyanine Pc 4 was tested as a photosensitizer for the selective photoinactivation of malignant cells in bone marrow transplantation samples.. Using a murine model system, incubation of 1.5 3107 cells/mL with 15 nM Pc 4 followed by exposure to red light (l.600 nm, fluence of 18 J/cm2) was shown to result in a greater than 6 log10 reduction of the clonogenic growth for the murine cell lines ABE8.1/2, BC3A and L1210.. The clonogenic growth of WEHI-3 and P815 cells was reduced by more than 5 log10 and more than 3 log10 , respectively.

Article Title: Mobilization of opioid-containing polymorphonuclear cells by hematopoietic growth factors and influence on inflammatory pain.
Article Snippet: Background: Leukocytes can control inflammatory pain by secretion of opioid peptides, stimulated by cold-water swimming or local injection of corticotropin-releasing factor, and subsequent activation of opioid receptors on peripheral sensory neurons.. This study investigated whether mobilization of polymorphonuclear cells (PMN) by granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) enhances immigration of opioid-containing PMN and peripheral opioid analgesia in rats with Freund complete adjuvant–induced hind paw inflammation.. Methods: In circulating PMN of rats treated with G-CSF SCF and sham-treated rats, opioid peptide content was measured by radioimmunoassay.

Article Title: In vivo expansion of transduced murine hematopoietic cells with a selective amplifier gene.
Article Snippet: Background Hematopoietic stem-cell-directed gene transfer has achieved limited success in transducing clinically relevant levels of target cells.. The expansion of gene-modified cells is one way to circumvent the problem of inefficient transduction with current vectors.. To this end, we have developed ‘selective amplifier genes’ (SAGs) that encode chimeric proteins that are a fusion of granulocyte colony-stimulating factor receptor and the steroidbinding domain.

Article Title: Polysialic acid, a glycan with highly restricted expression, is found on human and murine leukocytes and modulates immune responses.
Article Snippet: Colony-forming cell assays were performed as previously described (19) in serum-deprived medium with substitution of 10% FBS for low-density lipoprotein (HyClone Laboratories). .. Growth was supported by 100 ng/ml recombinant rat stem cell factor (Amgen) and 10 U/ml recombinant human (rh) erythropoietin (Amgen). ..



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